Intermediate

Part:BBa_K1647003:Design

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

B0034+pcyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a strong RBS to produce high levels of expression of the pcyA protein.


Source

Assembled from parts in the previous distribution kits.

References